RBRC03705 In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Cre recombinase [P1_ap003, Bacteriophage genomic DNA (class 1)], FRT sequence [yeast genomic DNA (class 1)], Nestin [mouse genomic DNA (neuronal-specific promoter)(class 1)], Phage P1 loxP derived from pBACe3.6 vector, BAC clone RP23-141A13, SV40 poly A, SV40 Large T antigen nuclear localization signal (NLS) <a href='https://brc.riken.jp/mus/pcr03705'>Genotyping protocol -PCR-</a> Cre/loxP system FLP/frt system Cre-driver transgenic mice. A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse nestin promoter fragment derived from the BAC library (BAC clone RP24-64E17)was microinjected into C57BL/6NCrlCrlj fertilized eggs. Tissue-specific expression of Cre recombinase has not been examined yet. Users are encouraged to test the tissue-specificity and feed back the data for the research community. C（3〜6か月） Nestin-NLSCre Nestin-NLSCre Carrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown). C (3-6 months) RIKEN BRC RIKEN BRC C57BL/6N-Tg(Nes-cre)52Utr/Rbrc C57BL/6N-Tg(Nes-cre)52Utr/Rbrc Developed by Laboratory Animal Resource Center, Tsukuba University and RIKEN BioResource Center in 2009. C57BL/6N background. 条件を付加する。<br>研究成果の公表にあたっては、八神健一（筑波大学生命科学動物資源センター）と理研バイオリソースセンターに対して謝辞の表明を必要とする。研究成果の公表にあたって寄託者の指定する文献を引用する(後日指定)。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 true